纤毛内微管蛋白通过 IFT74 和 IFT81 运输的分子基础。
Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.
机构信息
Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
出版信息
Science. 2013 Aug 30;341(6149):1009-12. doi: 10.1126/science.1240985.
Abstract
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
摘要
纤毛前体（如微管蛋白）从细胞质到纤毛尖端的鞭毛内运输（IFT）参与了纤毛的构建，纤毛是大多数真核细胞上的毛发状细胞器。然而，IFT 的分子机制还不清楚。在这里，我们发现两个核心 IFT 蛋白 IFT74 和 IFT81 形成一个微管蛋白结合模块，并将相互作用定位到 IFT81 的钙调蛋白同源结构域和 IFT74 的高度碱性结构域。IFT81 的敲低和点突变的挽救实验表明，IFT81 的微管蛋白结合对于人细胞中的纤毛发生是必需的。
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