Pazour Gregory J, Agrin Nathan, Leszyk John, Witman George B
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
J Cell Biol. 2005 Jul 4;170(1):103-13. doi: 10.1083/jcb.200504008.
Cilia and flagella are widespread cell organelles that have been highly conserved throughout evolution and play important roles in motility, sensory perception, and the life cycles of eukaryotes ranging from protists to humans. Despite the ubiquity and importance of these organelles, their composition is not well known. Here we use mass spectrometry to identify proteins in purified flagella from the green alga Chlamydomonas reinhardtii. 360 proteins were identified with high confidence, and 292 more with moderate confidence. 97 out of 101 previously known flagellar proteins were found, indicating that this is a very complete dataset. The flagellar proteome is rich in motor and signal transduction components, and contains numerous proteins with homologues associated with diseases such as cystic kidney disease, male sterility, and hydrocephalus in humans and model vertebrates. The flagellum also contains many proteins that are conserved in humans but have not been previously characterized in any organism. The results indicate that flagella are far more complex than previously estimated.
纤毛和鞭毛是广泛存在的细胞器，在整个进化过程中高度保守，在从原生生物到人类的真核生物的运动、感官感知和生命周期中发挥着重要作用。尽管这些细胞器普遍存在且很重要，但其组成尚不为人所知。在这里，我们使用质谱法鉴定来自莱茵衣藻纯化鞭毛中的蛋白质。高可信度鉴定出360种蛋白质，中等可信度鉴定出另外292种蛋白质。在101种先前已知的鞭毛蛋白中发现了97种，表明这是一个非常完整的数据集。鞭毛蛋白质组富含运动和信号转导成分，并且包含许多与人类和模式脊椎动物的疾病（如多囊肾病、男性不育症和脑积水）相关的具有同源物的蛋白质。鞭毛还包含许多在人类中保守但以前在任何生物体中都未被表征的蛋白质。结果表明，鞭毛比以前估计的要复杂得多。
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