Sutula Justyna, Coulthwaite Lisa, Thomas Linda, Verran Joanna
Microbiology Research Group, School of Health Care Science, Manchester Metropolitan University, Manchester, UK.
Microb Ecol Health Dis. 2012 Oct 3;23. doi: 10.3402/mehd.v23i0.18404. eCollection 2012.
It is acknowledged that oral and general health status declines with age. The global population of denture wearers is increasing, so is the incidence of denture biofilm-related problems, such as denture-associated stomatitis, aspiration pneumonia and malodour. It has been suggested that consumption of probiotic bacteria may improve oral health. However, the effects of probiotics on the oral microbiota of denture wearers have received little attention.
The aim of this study was to investigate the effect of consumption of a commercial probiotic product (Yakult) on microbiota of saliva, tongue and denture biofilm in healthy complete denture wearers. Eight healthy complete denture-wearing National Health Service (NHS) patients undertook a 7-week trial comprising three phases: baseline; 4-week consumption of one bottle of Yakult per day, each containing a minimum 6.5×10(9) viable cells of Lactobacillus casei strain Shirota (LcS); 4-week washout period. The microbial viability and composition of saliva, tongue dorsum coating and denture biofilm were assessed using a range of solid selective and indicator media. Questionnaires were used to explore participants' denture cleaning habits and impact of wearing dentures on their life quality and well-being [modified oral health impact profile (OHIP-14)] prior to and after the study.
Seven volunteers (1 male, 6 females) completed the trial. LcS temporarily colonised oral cavity and denture surface. There was no significant change in the viability of Streptococcus mutans, acidogenic microorganisms, total anaerobic species and Gram-negative obligate anaerobes between study phases. There was no obvious effect of LcS on occurrence and viability of Candida. Participants presented a good general knowledge of denture hygiene and their responses to OHIP-14 questionnaires improved after completing the study (p=0.16).
It appeared that 4-week consumption of probiotic drink had no overall effect on selected oral parameters in healthy denture wearers despite temporary presence of LcS.
人们公认，口腔和总体健康状况会随着年龄增长而下降。全球佩戴假牙的人口在增加，与假牙生物膜相关问题的发生率也在上升，比如义齿性口炎、吸入性肺炎和口臭。有人提出，食用益生菌可能会改善口腔健康。然而，益生菌对假牙佩戴者口腔微生物群的影响却很少受到关注。
本研究的目的是调查食用一种商业益生菌产品（养乐多）对健康全口假牙佩戴者唾液、舌头和假牙生物膜微生物群的影响。八名佩戴全口假牙的英国国家医疗服务体系（NHS）健康患者进行了一项为期7周的试验，该试验包括三个阶段：基线期；每天饮用一瓶养乐多，持续4周，每瓶至少含有6.5×10⁹ 干酪乳杆菌代田株（LcS）的活细胞；4周的洗脱期。使用一系列固体选择性培养基和指示培养基评估唾液、舌背涂层和假牙生物膜的微生物活力及组成。在研究前后，通过问卷调查来探究参与者的假牙清洁习惯以及佩戴假牙对其生活质量和幸福感的影响[改良口腔健康影响量表（OHIP - 14）]。
七名志愿者（1名男性，6名女性）完成了试验。LcS暂时定殖于口腔和假牙表面。在各研究阶段之间，变形链球菌、产酸微生物、总厌氧菌和革兰氏阴性专性厌氧菌的活力没有显著变化。LcS对念珠菌的发生和活力没有明显影响。参与者对假牙卫生有较好的总体认识，并且在完成研究后，他们对OHIP - 14问卷的回答有所改善（p = 0.16）。
尽管LcS暂时存在，但似乎食用4周益生菌饮料对健康假牙佩戴者的选定口腔参数没有总体影响。
