Kirchhoefer R D, Wells C E
J Assoc Off Anal Chem. 1975 Sep;58(5):879-83.
Two semiautomated analytical systems have been developed to determine ergonovine and methylergonovine maleate in single tablets and injections. The active ingredient is dissolved in a tartrate buffer. In the fluorometric method the stream is automatically diluted with tartrate buffer and excited with ultraviolet light; the resulting fluorescence is measured and recorded. In the colorimetric method, the stream is automatically made basic with sodium hydroxide and extracted with n-butanol. The extract is mixed with p-dimethylaminobenzaldehyde reagent. After reaction, the solution is mixed with sodium nitrite solution, and the developed color is measured at 550 nm. Recoveries of 100% were obtained from spiked placebos. Standard deviations for powdered tablet and injection samples ranged from 0.63 to 1.24%. Comparisons with the USP XVIII methods are presented.
已开发出两种半自动分析系统，用于测定单片制剂和注射剂中的麦角新碱和马来酸甲基麦角新碱。活性成分溶解于酒石酸盐缓冲液中。在荧光法中，液流用酒石酸盐缓冲液自动稀释，并用紫外光激发；测量并记录产生的荧光。在比色法中，液流用氢氧化钠自动碱化，并用正丁醇萃取。萃取液与对二甲氨基苯甲醛试剂混合。反应后，溶液与亚硝酸钠溶液混合，并在550nm处测量显色。加标安慰剂的回收率为100%。片剂粉末和注射剂样品的标准偏差范围为0.63%至1.24%。文中还与美国药典第十八版方法进行了比较。
