Brower J F
J Assoc Off Anal Chem. 1977 Jan;60(1):27-31.
A semiautomated colorimetric method for the determination of prednisolone and prednisone was collaboratively studied by 6 collaborators. In the method, an alcoholic solution of the drug is extracted with chloroform and the extract is reacted with tetramethylammonium hydroxide and blue tetrazolium; the absorbance of the resulting color is read at 525 nm. Collaborators were supplied with 4 composites of tablets of different dosage levels, 2 containing prednisolone and 2 containing prednisone. Results agreed with those obtained by the author using the USP total steroid assay method. The coefficients of variation of the individual collaborator's results for prednisolone and prednisone ranged from 0.54 to 2.38 and from 0.34 to 2.19%, respectively. This method has been adopted as official first action.
6位合作者共同研究了一种用于测定泼尼松龙和泼尼松的半自动比色法。在该方法中，药物的乙醇溶液用氯仿萃取，萃取液与氢氧化四甲铵和蓝四氮唑反应；在525nm处读取所得颜色的吸光度。向合作者提供了4种不同剂量水平的片剂组合，其中2种含泼尼松龙，2种含泼尼松。结果与作者使用美国药典总类固醇测定法获得的结果一致。各合作者对泼尼松龙和泼尼松测定结果的变异系数分别为0.54%至2.38%和0.34%至2.19%。该方法已被采用为暂行首次法定方法。
