Ben-Zaken S, Eliakim A, Nemet D, Kassem E, Meckel Y
Genetics and Molecular Biology Laboratory, The Zinman College for Physical Education and Sport Sciences at the Wingate Institute , Netanya , Israel.
Free Radic Res. 2013 Dec;47(12):1002-8. doi: 10.3109/10715762.2013.838627. Epub 2013 Sep 24.
The purpose of the current study was to determine the frequency distribution of manganese superoxide dismutase (MnSOD) Val-9Ala polymorphism (rs1799725) among 195 trained endurance and power athletes and 240 healthy controls. Genomic DNA was extracted using a standard protocol. Genotyping of the MnSOD Val-9Ala polymorphism was performed using polymerase chain reaction (PCR). Results showed a higher proportion of the Val/Ala and Ala/Ala genotype, and a lower proportion of Val/Val genotype, in the athletes group compared with that of the controls. The Ala allele frequency was significantly higher (p < 0.001) in the athletes group (46%) compared with that in the control (29%). Interestingly, there was no difference between the endurance and power athletes. In addition, the frequency of Ala/Ala genotype was significantly higher (p < 0.05) among top (international and Olympic-level) athletes (29%) compared with that among national-level endurance and power athletes (17%). We conclude that 1) the Ala allele is more frequent in athletes than in controls; and 2) the higher frequency of the Ala allele was noted in both endurance and power athletes compared with that in controls, suggesting that the positive association between the Ala allele and athletic performance may be related to ROS-related angiogenesis, mitochondrial biosynthesis, and muscle hypertrophy, and not to MnSOD aerobic properties.
本研究的目的是确定195名耐力和力量项目的训练有素的运动员以及240名健康对照者中锰超氧化物歧化酶（MnSOD）Val-9Ala多态性（rs1799725）的频率分布。使用标准方案提取基因组DNA。采用聚合酶链反应（PCR）对MnSOD Val-9Ala多态性进行基因分型。结果显示，与对照组相比，运动员组中Val/Ala和Ala/Ala基因型的比例更高，而Val/Val基因型的比例更低。运动员组中Ala等位基因频率（46%）显著高于对照组（29%）（p<0.001）。有趣的是，耐力和力量项目的运动员之间没有差异。此外，顶级（国际和奥运水平）运动员中Ala/Ala基因型的频率（29%）显著高于国家级耐力和力量项目运动员（17%）（p<0.05）。我们得出结论：1）运动员中Ala等位基因的频率高于对照组；2）与对照组相比，耐力和力量项目的运动员中Ala等位基因的频率均更高，这表明Ala等位基因与运动表现之间的正相关可能与活性氧相关的血管生成、线粒体生物合成和肌肉肥大有关，而与MnSOD的有氧特性无关。
