Conservation Biology Division, Northwest Fisheries Science Center, 2725 Montlake Blvd East, Seattle, WA 98112, USA.
Mol Ecol Resour. 2014 Jan;14(1):209-14. doi: 10.1111/1755-0998.12157. Epub 2013 Aug 31.
NeEstimator v2 is a completely revised and updated implementation of software that produces estimates of contemporary effective population size, using several different methods and a single input file. NeEstimator v2 includes three single-sample estimators (updated versions of the linkage disequilibrium and heterozygote-excess methods, and a new method based on molecular coancestry), as well as the two-sample (moment-based temporal) method. New features include the following: (i) an improved method for accounting for missing data; (ii) options for screening out rare alleles; (iii) confidence intervals for all methods; (iv) the ability to analyse data sets with large numbers of genetic markers (10 000 or more); (v) options for batch processing large numbers of different data sets, which will facilitate cross-method comparisons using simulated data; and (vi) correction for temporal estimates when individuals sampled are not removed from the population (Plan I sampling). The user is given considerable control over input data and composition, and format of output files. The freely available software has a new JAVA interface and runs under MacOS, Linux and Windows.
NeEstimator v2 是一款经过全面修订和更新的软件，可使用多种不同方法和单个输入文件来估算当代有效种群规模。NeEstimator v2 包括三个单样本估算器（连锁不平衡和杂合子过剩方法的更新版本，以及一种基于分子亲缘关系的新方法），以及两个样本（基于矩的时变）方法。新功能包括以下内容：（i）一种改进的缺失数据处理方法；（ii）筛选稀有等位基因的选项；（iii）所有方法的置信区间；（iv）分析具有大量遗传标记（10000 个或更多）的数据集的能力；（v）批量处理大量不同数据集的选项，这将有助于使用模拟数据进行跨方法比较；（vi）当从群体中去除采样个体时对时间估算的校正（计划 I 采样）。用户可以对输入数据和组成以及输出文件的格式进行相当大的控制。该免费软件具有新的 JAVA 接口，可在 MacOS、Linux 和 Windows 下运行。
