Tu Jianfeng, Si Fangfang, Wu Qiong, Cong Bo, Xing Xiumei, Yang Fu He
Institute of Special Wild Economic Animal and Plant Science, Chinese Academy Agricultural Sciences , Jilin, Jilin Province , PR China .
Mitochondrial DNA. 2014 Apr;25(2):102-3. doi: 10.3109/19401736.2013.784756. Epub 2013 Aug 31.
Muscovy duck (Cairina moschata) is the most popular meat duck, and it can be crossed with most breeds of domestic ducks in captivity to produce sterile hybrids. In this study, complete mitochondrial genome of the Muscovy duck was sequenced by long and accurate polymerase chain reaction (LA-PCR) as well as the primer walking sequence method. The entire mitochondrial genome of Muscovy duck was 16,610 bp in length, gene composition and arrangement conformed to most bird, which contained the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and a non-coding region. The characteristic of the mitochondrial genome was analyzed in detail. Our complete mitochondrial genome sequence will be useful for phylogenetics, and be available as basic data for the breeding and genetics.
番鸭（Cairina moschata）是最受欢迎的肉鸭品种，在圈养条件下它能与大多数家鸭品种杂交产生不育杂种。在本研究中，采用长片段精确聚合酶链反应（LA-PCR）和引物步移测序法对番鸭的线粒体全基因组进行了测序。番鸭线粒体全基因组长度为16,610 bp，基因组成和排列与大多数鸟类一致，包含22个tRNA、2个rRNA、13个蛋白质编码基因和一个非编码区的典型结构。对线粒体基因组的特征进行了详细分析。我们的线粒体全基因组序列将有助于系统发育研究，并可作为育种和遗传学的基础数据。
