Huang Y S, Mills D E, Ward R P, Simmons V A, Horrobin D F
Efamol Research Institute, Kentville, Nova Scotia, Canada.
Proc Soc Exp Biol Med. 1990 Oct;195(1):136-41. doi: 10.3181/00379727-195-43132.
The effects of dietary cholesterol (CH) and isolation stress on fatty acid compositions of plasma and liver cholesteryl ester and phospholipids were compared in growing rats fed an 18:2n-6 or an 18:3n-6 enriched semisynthetic diet for 2 weeks. Stress, CH-feeding, and dietary fats had no significant effects on plasma CH level, but CH-feeding alone elevated the liver CH concentrations. CH-feeding also modulated the liver polyunsaturated fatty acid compositions, i.e., increasing 18:2n-6 levels, and reducing 20:4n-6 levels, indicating an inhibition of the enzymes, delta-6 and delta-5-desaturases. The extent of these changes was less in rats fed 18:3n-6 than in those fed 18:2n-6. Stress, which alone had no significant effects on plasma and liver fatty acid compositions, attenuated the CH-induced changes of fatty acid levels.
在以富含18:2n-6或18:3n-6的半合成饲料喂养2周的生长大鼠中，比较了膳食胆固醇（CH）和隔离应激对血浆、肝脏胆固醇酯和磷脂脂肪酸组成的影响。应激、CH喂养和膳食脂肪对血浆CH水平无显著影响，但仅CH喂养会提高肝脏CH浓度。CH喂养还调节了肝脏多不饱和脂肪酸组成，即增加18:2n-6水平并降低20:4n-6水平，表明对δ-6和δ-5-去饱和酶有抑制作用。这些变化的程度在喂食18:3n-6的大鼠中比在喂食18:2n-6的大鼠中要小。单独应激对血浆和肝脏脂肪酸组成无显著影响，但减弱了CH诱导的脂肪酸水平变化。
