Nunes Erika Takagi, Braga Adriane Araújo, Camargo-Mathias Maria Izabel
Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n(o), Alegre, ES 29500-000, Brazil.
Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n(o), Alegre, ES 29500-000, Brazil.
Acta Histochem. 2014 Jan;116(1):243-51. doi: 10.1016/j.acthis.2013.07.011. Epub 2013 Aug 29.
This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts.
本研究提供了成年雌性巴西粉红对虾（Farfantepenaeus brasiliensis）在两个不同发育阶段（性腺发育成熟阶段和性腺耗尽阶段）肝胰腺细胞的组织化学数据。巴西粉红对虾雌虾采自巴西圣埃斯皮里图州瓜拉帕里海岸的海水区域。在这个消化腺中鉴定出了五种细胞类型：B（泡状）、E（胚胎型）、F（纤维状）、M（基底型）和R（吸收型）。采用以下技术对消化腺进行染色：PAS/阿尔辛蓝（用于多糖）、溴酚蓝（用于蛋白质）、冯·科萨法（用于结合钙）和贝克法（用于脂质）。在R细胞的液泡内发现了酸性糖缀合物，而中性多糖存在于B细胞中以及微绒毛附近。在性腺耗尽的雌虾中，在肾小管间隙和肾小管管腔内也可见到多糖。F细胞和M细胞中蛋白质含量最高，在R细胞以及B细胞的液泡内也有大量蛋白质。结合钙主要存在于F细胞和M细胞中。F细胞对脂质呈强阳性染色，而R细胞仅呈弱阳性染色。E细胞对所应用的任何染色技术均无反应。与性腺耗尽的雌虾相比，性腺发育成熟的雌虾肝胰腺细胞组织化学组成的相似性，其原因在于这些元素仅在巴西粉红对虾性腺发育的初始阶段大量转移至卵母细胞中。此外，它们可能与蜕皮阶段关系更大，就像钙盐的情况一样。
