Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
Food Chem. 2013 Dec 15;141(4):3954-9. doi: 10.1016/j.foodchem.2013.06.098. Epub 2013 Jul 2.
In this work were extracted bioactive compounds from Brassica oleracea var capitata using supercritical CO2 and evaluated the antioxidant potential of the extracts. Five extractions were accomplished to investigate the influence of pressure (10-25 MPa) and temperature (20-60 °C) in the extraction yield, chemical composition and antioxidant potential towards peroxyl, superoxide and hydroxyl radicals. The highest extraction yield was obtained at 60 °C and 25 MPa, which was 0.47 wt% (run 2). In the characterisation of the extracts obtained was possible the identification of sulforaphane and iberin nitrile that present known biological properties. The extracts of all runs presented antioxidant activities towards the three radicals, but the highest activities for all radicals were using the extracts obtained in the run 2. The use of supercritical CO2 extraction to obtain bioactive compounds of B. oleracea var capitata showed to be a promising alternative to conventional extraction methods, since allowed the extraction of compounds with scientific and industrial interest.
本工作使用超临界 CO2 从甘蓝型油菜中提取生物活性化合物，并评估了提取物的抗氧化潜力。进行了五次萃取，以研究压力（10-25 MPa）和温度（20-60°C）对萃取产率、化学成分和对过氧自由基、超氧自由基和羟基自由基的抗氧化潜力的影响。在 60°C 和 25 MPa 下获得了最高的萃取产率，为 0.47 wt%（运行 2）。在对获得的提取物进行的表征中，鉴定出具有已知生物特性的萝卜硫素和白藜芦醇腈。所有运行的提取物均对三种自由基表现出抗氧化活性，但使用运行 2 中获得的提取物对所有自由基的活性最高。使用超临界 CO2 萃取从甘蓝型油菜中获得生物活性化合物，这是一种有前途的替代传统萃取方法的方法，因为它允许提取具有科学和工业价值的化合物。
