Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Food Chem. 2013 Dec 15;141(4):4046-50. doi: 10.1016/j.foodchem.2013.06.117. Epub 2013 Jul 4.
A rapid quantification of seven ginsenosides in Du Shen Tang decoction, the decoction of the root of Panax ginseng was investigated by rapid resolution liquid chromatography coupled with tandem mass spectrometry (RRLC-MS/MS). All analytes including seven major constituents, namely ginsenoside-Re, Rg1, Rb1, Rc, Rb2, Rd and Rf were analysed simultaneously in short time and provide good linearity (r>0.9976), repeatability (RSD<5.67%), intra- and inter-day precisions (RSD<5.06%) with accuracies (94.65-105.31%) and recovery (93.76-106.82%) by using optimised methods. In addition, the content of seven ginsenosides in the decoction prepared by the root of 2-year-old, 4-year-old and 6-year-old Panax ginseng was also analysed. The results showed the concentration of ginsenosides detected in Du Shen Tang decoction was affected by the difference in collected times of the root of Panax ginseng. The established method could be used as a rapid and reliable approach for assessment of the quantity of Du Shen Tang decoction.
采用快速分辨液相色谱-串联质谱法（RRLC-MS/MS）对参芪汤（人参根的汤剂）中 7 种人参皂苷进行了快速定量分析。在短时间内同时分析了所有分析物，包括 7 种主要成分，即人参皂苷-Re、Rg1、Rb1、Rc、Rb2、Rd 和 Rf，均具有良好的线性（r>0.9976）、重复性（RSD<5.67%）、日内和日间精密度（RSD<5.06%），准确度（94.65-105.31%）和回收率（93.76-106.82%）。此外，还分析了由 2 年、4 年和 6 年生人参根制备的汤剂中 7 种人参皂苷的含量。结果表明，参芪汤中人参皂苷的浓度受人参根采集时间的差异影响。所建立的方法可作为评估参芪汤用量的快速可靠方法。
