School of Chemical Sciences & Food Technology, Faculty of Science & Technology, 43600 UKM, Bangi Selangor, Malaysia.
Food Chem. 2013 Dec 15;141(4):4102-6. doi: 10.1016/j.foodchem.2013.06.112. Epub 2013 Jul 3.
A stable chromogenic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) is commonly used for the determination of antioxidant activity. In this paper, DPPH was dried into 96 well microplate to produce DPPH dry reagent array plate, based on which the highly sensitive and high throughput determination of antioxidant activities was achieved. The spectrophotometric characterization of the microplate containing dried or fresh DPPH free radicals was reported. The response of the DPPH dry reagent array towards different standard antioxidants was studied. The reaction for DPPH in fresh or dry reagent array with Trolox was reported and compared. The DPPH dry reagent array was used to study the antioxidant activity of banana, green tea, pink guava, and honeydew and the results were compared to the samples reacted with freshly prepared DPPH. The proposed method is comparable to the classical DPPH method, more convenient, simple to operate with minimal solvent required and excellent sensitivity.
稳定的显色自由基 2,2-二苯基-1-苦基肼（DPPH）常用于测定抗氧化活性。本文将 DPPH 干燥成 96 孔微孔板，制成 DPPH 干试剂阵列板，基于此，实现了高灵敏度和高通量的抗氧化活性测定。报道了含有干燥或新鲜 DPPH 自由基的微孔板的分光光度特性。研究了 DPPH 干试剂阵列对不同标准抗氧化剂的响应。报道并比较了 Trolox 在新鲜或干燥的试剂阵列中与 DPPH 的反应。将 DPPH 干试剂阵列用于研究香蕉、绿茶、粉红番石榴和蜜瓜的抗氧化活性，并将结果与与新鲜制备的 DPPH 反应的样品进行比较。该方法与经典的 DPPH 方法相当，更方便、操作简单，所需溶剂最少，灵敏度高。
