Guild Associates, Inc., Charleston, SC 29407, United States.
J Microbiol Methods. 2013 Nov;95(2):156-61. doi: 10.1016/j.mimet.2013.08.013. Epub 2013 Aug 28.
Genetically modified phages have the potential to detect pathogenic bacteria from clinical, environmental, or food-related sources. Herein we assess an engineered 'bioluminescent' reporter phage (Wß::luxAB) as a clinical diagnostic tool for Bacillus anthracis, the etiological agent of anthrax. Wß::luxAB is able to rapidly (within minutes) detect a panel of B. anthracis strains by transducing a bioluminescent phenotype. The reporter phage displays species specificity by its inability, or significantly reduced ability, to detect members of the closely related Bacillus cereus group and other common bacterial pathogens. Using spiked clinical specimens, Wß::luxAB detects B. anthracis within 5 h at clinically relevant concentrations, and provides antibiotic susceptibility information that mirrors the CLSI method, except that data are obtained at least 5-fold faster. Although anthrax is a treatable disease, a positive patient prognosis is dependent on timely diagnosis and appropriate therapy. Wß::luxAB rapidly detects B. anthracis and determines antibiotic efficacy, properties that will help patient outcome.
基因改造噬菌体有可能从临床、环境或与食品相关的来源中检测致病细菌。在此，我们评估了一种经过工程改造的“生物发光”报告噬菌体（Wß::luxAB）作为炭疽病病原体炭疽杆菌的临床诊断工具。Wß::luxAB 能够通过转导生物发光表型，快速（几分钟内）检测一系列炭疽杆菌菌株。报告噬菌体通过其不能或显著降低的能力来检测密切相关的蜡样芽孢杆菌组和其他常见细菌病原体的成员，从而显示出种特异性。使用添加的临床标本，Wß::luxAB 在临床相关浓度下 5 小时内即可检测到炭疽杆菌，并提供与 CLSI 方法相似的抗生素敏感性信息，只是数据获取速度快 5 倍以上。尽管炭疽病是一种可治疗的疾病，但患者的预后取决于及时的诊断和适当的治疗。Wß::luxAB 可快速检测炭疽杆菌并确定抗生素疗效，这些特性将有助于改善患者的预后。
