Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
Gene. 2013 Nov 10;530(2):185-92. doi: 10.1016/j.gene.2013.08.048. Epub 2013 Aug 27.
Flag leaf is one of the key photosynthesis organs during rice reproductive stage. A time course microarray analysis of rice flag leaf was done after 40°C treatment for 0 min, 20 min, 60 min, 2h, 4h, and 8h. The identified significant heat responsive genes were mainly involved in transcriptional regulation, transport, protein binding, antioxidant, and stress response. KMC analysis discovered the time-dependent gene expression pattern under heat. MapMan analysis demonstrated that, under heat treatment, Hsp genes and genes involved in glycolysis and ubiquitin-proteasome were enhanced, and genes involved in TCA, carotenoid, dihydroflavonol and anthocyanin metabolisms and light-reaction in the photosynthesis were widely repressed. Meanwhile, some rate-limiting enzyme genes in shikimate, lignin, and mevalonic acid metabolisms were up-regulated, revealing the importance of maintaining specific secondary metabolites under heat stress. The present study increased our understanding of heat response in rice flag leaf and provided good candidate genes for crop improvement.
旗叶是水稻生殖期的主要光合作用器官之一。对水稻旗叶进行了 40°C 处理 0min、20min、60min、2h、4h 和 8h 的时间进程微阵列分析。鉴定出的显著热响应基因主要涉及转录调控、运输、蛋白结合、抗氧化和应激反应。KMC 分析发现了热下的时程基因表达模式。MapMan 分析表明，在热处理下，Hsp 基因和参与糖酵解和泛素-蛋白酶体的基因增强，而参与三羧酸循环、类胡萝卜素、二氢黄酮醇和花色苷代谢以及光合作用中光反应的基因广泛受到抑制。同时，一些莽草酸、木质素和甲羟戊酸代谢中的限速酶基因上调，表明在热胁迫下维持特定次生代谢物的重要性。本研究增加了我们对水稻旗叶热响应的理解，并为作物改良提供了良好的候选基因。
