Rašić Mišić Ivana, Miletić Gordana, Mitić Snežana, Mitić Milan, Pecev-Marinković Emilija
Department of Chemistry, Faculty of Sciences and Mathematics, University of Niš
Chem Pharm Bull (Tokyo). 2013;61(9):913-9. doi: 10.1248/cpb.c13-00197.
The new kinetically-based spectrophotometric method for the determination of microquantities of ampicillin is proposed in the present paper. Ampicillin degradation in strong alkaline medium was applied for the method development. The reaction rate was monitored at 265 nm. A differential variation of the tangent method was used to process the kinetic data. The method is valid over the 3.49-55.84 µg/mL ampicillin concentration interval with relative standard deviation (RSD) range 7.79-3.20%. The calculated detection limit was determined at 2.58 µg/mL based on the 3.3S0 criterion. The interference effects of some metal ions, anions, amino acids and other molecules were investigated in order to assess the method selectivity. The method was successfully applied to determining the content of ampicillin in commercial pharmaceutical preparations and human urine. The obtained results were in good correlation with the HPLC method results. The newly developed method is simple, inexpensive and efficient for the analysis of a large number of samples at room temperature in a short time.
本文提出了一种基于动力学的测定微量氨苄西林的分光光度法。该方法利用氨苄西林在强碱性介质中的降解反应进行开发。在265nm处监测反应速率。采用切线法的微分变化处理动力学数据。该方法在3.49 - 55.84μg/mL氨苄西林浓度范围内有效，相对标准偏差（RSD）为7.79 - 3.20%。根据3.3S0标准计算得出的检测限为2.58μg/mL。研究了一些金属离子、阴离子、氨基酸和其他分子的干扰效应，以评估该方法的选择性。该方法成功应用于测定市售药物制剂和人尿中氨苄西林的含量。所得结果与高效液相色谱法结果具有良好的相关性。新开发的方法简单、廉价且高效，可在室温下短时间内对大量样品进行分析。
