Lewis Rebecca, May Hannah, Mobasheri Ali, Barrett-Jolley Richard
Musculoskeletal Biology; Institute of Ageing and Chronic Disease; Faculty of Health & Life Sciences; University of Liverpool; Liverpool, UK; The D-BOARD European Consortium for Biomarker Discovery.
Musculoskeletal Biology; Institute of Ageing and Chronic Disease; Faculty of Health & Life Sciences; University of Liverpool; Liverpool, UK.
Channels (Austin). 2013 Nov-Dec;7(6):459-67. doi: 10.4161/chan.26071. Epub 2013 Aug 30.
To date, a range of ion channels have been identified in chondrocytes using a number of different techniques, predominantly electrophysiological and/or biomolecular; each of these has its advantages and disadvantages. Here we aim to compare and contrast the data available from biophysical and microarray experiments. This letter analyses recent transcriptomics datasets from chondrocytes, accessible from the European Bioinformatics Institute (EBI). We discuss whether such bioinformatic analysis of microarray datasets can potentially accelerate identification and discovery of ion channels in chondrocytes. The ion channels which appear most frequently across these microarray datasets are discussed, along with their possible functions. We discuss whether functional or protein data exist which support the microarray data. A microarray experiment comparing gene expression in osteoarthritis and healthy cartilage is also discussed and we verify the differential expression of 2 of these genes, namely the genes encoding large calcium-activated potassium (BK) and aquaporin channels.
迄今为止，利用多种不同技术，主要是电生理学和/或生物分子技术，已在软骨细胞中鉴定出一系列离子通道；每种技术都有其优缺点。在此，我们旨在比较和对比来自生物物理和微阵列实验的现有数据。这篇信函分析了来自欧洲生物信息学研究所（EBI）的软骨细胞近期转录组学数据集。我们讨论了对微阵列数据集进行此类生物信息学分析是否有可能加速软骨细胞中离子通道的识别和发现。讨论了在这些微阵列数据集中出现频率最高的离子通道及其可能的功能。我们还讨论了是否存在支持微阵列数据的功能或蛋白质数据。此外，还讨论了一项比较骨关节炎和健康软骨中基因表达的微阵列实验，并验证了其中两个基因的差异表达，即编码大电导钙激活钾（BK）通道和水通道蛋白通道的基因。
