Abbineni Prabhodh S, Hibbert Julie E, Coorssen Jens R
Department of Molecular Physiology, School of Medicine, University of Western Sydney, NSW, Australia.
Biol Bull. 2013 Aug;224(3):200-17. doi: 10.1086/BBLv224n3p200.
Regulated exocytosis is one of the defining features of eukaryotic cells, underlying many conserved and essential functions. Definitively assigning specific roles to proteins and lipids in this fundamental mechanism is most effectively accomplished using a model system in which distinct stages of exocytosis can be effectively separated. Here we discuss the establishment of sea urchin cortical vesicle fusion as a model to study regulated exocytosis-a system in which the docked, release-ready, and late Ca(2+)-triggered steps of exocytosis are isolated and can be quantitatively assessed using the rigorous coupling of functional and molecular assays. We provide an overview of the insights this has provided into conserved molecular mechanisms and how these have led to and integrate with findings from other regulated exocytotic cells.
受调控的胞吐作用是真核细胞的标志性特征之一，是许多保守且基本功能的基础。在这一基本机制中，要明确赋予蛋白质和脂质特定作用，最有效的方法是使用一个能有效分离胞吐作用不同阶段的模型系统。在此，我们讨论将海胆皮质囊泡融合作为研究受调控胞吐作用模型的建立——在这个系统中，胞吐作用的对接、准备释放以及后期钙离子触发步骤被分离出来，并且可以通过功能分析与分子分析的严格结合进行定量评估。我们概述了由此获得的关于保守分子机制的见解，以及这些见解如何引出并与其他受调控胞吐细胞的研究结果相结合。
