Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA.
Nat Chem Biol. 2013 Nov;9(11):671-3. doi: 10.1038/nchembio.1334. Epub 2013 Sep 1.
Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.
mRNA 的多聚腺苷酸化会导致蛋白质表达增加，以响应各种刺激，但很难确定在活细胞中发生多聚腺苷酸化的 mRNA。在这里，我们描述了一种可与点击化学兼容的核苷类似物，它可被选择性地掺入细胞中转录物的多(A)尾巴中。标记的 mRNA 的下一代测序可提供全转录组范围内的多聚腺苷酸化谱，并深入了解与多聚腺苷酸化相关的 mRNA 序列元件。
