Bikard David, Mazel Didier
Bacterial Genome Plasticity Unit, Institut Pasteur, Paris, France.
Methods Mol Biol. 2013;1073:169-74. doi: 10.1007/978-1-62703-625-2_14.
The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.
即使是小型基因网络的复杂性也使得它们很难进行合理设计。因此，在包括代谢工程在内的许多应用中，在定向进化过程中测试遗传元件的随机组合是很有意义的。在这里，我们描述了如何利用1类整合子的重组机制在大肠杆菌的染色体位点传递和改组遗传元件。
