Zupańska B, Brojer E, McIntosh J, Seyfried H, Howell P
Institute of Haematology, Warsaw, Poland.
Vox Sang. 1990;58(4):276-80. doi: 10.1111/j.1423-0410.1990.tb04999.x.
Comparisons have been made between the serological and immunological characteristics of 42 blood group alloantibodies (other than D) covering twelve systems using a monocyte-monolayer assay (MMA), a radiometric antiglobulin test for antibody binding and IgG subclass determinations. The results of the MMA correlated well with the level of IgG molecules bound on incompatible cells, and the highest levels in both cases were associated with the presence of the IgG3 subclass. However, limited clinical data shows that, while in general the MMA clearly identifies the clinically significant antibodies, the correlation with the degree of clinical outcome is less well defined, and in some instances other factors may be operating to ameliorate the in vivo effect of the antibody.
利用单核细胞单层试验（MMA）、用于抗体结合的放射免疫球蛋白试验以及IgG亚类测定，对涵盖12个系统的42种血型同种抗体（D抗体除外）的血清学和免疫学特征进行了比较。MMA的结果与不相容细胞上结合的IgG分子水平密切相关，在这两种情况下，最高水平都与IgG3亚类的存在有关。然而，有限的临床数据表明，虽然总体而言MMA能明确识别出具有临床意义的抗体，但与临床结果严重程度的相关性却不太明确，在某些情况下，可能有其他因素在减轻抗体的体内效应。
