Cassens U, Garritsen H S, Sibrowski W, Holzgreve W
Institut für Transfusionsmedizin, Westfälische Wilhelms-Universität Münster.
Geburtshilfe Frauenheilkd. 1996 Jun;56(6):297-300. doi: 10.1055/s-2007-1023234.
In haemolytic disease of newborn (erythroblastosis fetalis) the in vivo behaviour of erythrocytic IgG antibodies is of particular significance. The monocyte monolayer assay (MMA), which determines by microscopy the number of erythrocytes phagocytosed by monocytes, is an important functional test for the qualitative assessment of erythrocytic IgG antibodies. We set up a photometric MMA and compared it with the microscopic MMA. In both tests we employed commercially available rhesus antibody sera and examined 8 sera of pregnant women with mild and severe courses of haemolytic disease of newborn by the photometric MMA. Good agreement was found between the microscopic and photometric MMA (r = 0.93). Over and above this the photometric MMA correlated with the course of haemolytic disease of newborn in 7 out of 8 cases (with one false-positive finding). The photometric technique permits rapid, sensitive and reproducible determination of erythrophagocytosis in microtitre plates. This method is based on the photometric detection of haemoglobin of phagocytosed erythrocytes via a peroxidase reaction. Standardised photometric MMA could in future be more widely applied especially in haemolytic disease of newborn for the functional characterisation of erythrocytic antibodies, the incidence of intra-assay and inter-assay errors being low.
在新生儿溶血病（胎儿成红细胞增多症）中，红细胞IgG抗体的体内行为具有特殊意义。单核细胞单层试验（MMA）通过显微镜检查确定被单核细胞吞噬的红细胞数量，是对红细胞IgG抗体进行定性评估的一项重要功能测试。我们建立了一种光度法MMA，并将其与显微镜检查的MMA进行比较。在两项测试中，我们都使用了市售的恒河猴抗体血清，并通过光度法MMA检测了8例患有轻度和重度新生儿溶血病的孕妇血清。显微镜检查的MMA与光度法MMA之间具有良好的一致性（r = 0.93）。除此之外，光度法MMA在8例病例中的7例中与新生儿溶血病的病程相关（有1例假阳性结果）。光度技术可在微量滴定板中快速、灵敏且可重复地测定红细胞吞噬作用。该方法基于通过过氧化物酶反应对被吞噬红细胞的血红蛋白进行光度检测。标准化的光度法MMA未来可能会得到更广泛的应用，特别是在新生儿溶血病中用于红细胞抗体的功能表征，因为其批内和批间误差发生率较低。
