Sarkar Avik, Sengupta Dipanwita, Mandal Samir, Sen Gargi, Dutta Chowdhury Kaustav, Chandra Sadhukhan Gobinda
Genetics and Molecular Biology Laboratory, Vidyasagar College, 39 Sankar Ghosh Lane, Kolkata, 700006, India.
Environ Toxicol. 2015 Apr;30(4):396-410. doi: 10.1002/tox.21901. Epub 2013 Aug 30.
Sequelae of chronic lead (Pb(2+) ) toxicity includes anemia that is partially due to early death of erythrocytes characterized by excess accumulation of ROS and downregulation of antioxidant system causing oxidative stress and externalization of phosphatidylserine. In this study, pathophysiological based therapeutic application of garlic was evaluated against erythrocyte death. Results suggest that garlic administration prevents oxidative stress, restored the antioxidant balance in erythrocytes of Pb(2+) exposed mice. Moreover, in vitro studies revealed that activity of both scramblase and aminophospholipid translocase could be changed by modifying the critical sulfhydryl groups in presence of dithiothreitol during Pb(2+) exposure. Data also indicated that garlic treatment in Pb(2+) exposed mice exhibited sharp decline in PS exposure and increase in erythrocyte membrane thiol group followed by increase in aminophospholipid translocase activity and decline in scramblase activity. Findings indicated that garlic has the ability to restore the lifespan of erythrocytes during Pb(2+) exposure.
慢性铅（Pb(2+)）中毒的后遗症包括贫血，部分原因是红细胞过早死亡，其特征是活性氧过量积累、抗氧化系统下调，从而导致氧化应激和磷脂酰丝氨酸外化。在本研究中，评估了基于病理生理学的大蒜治疗对红细胞死亡的作用。结果表明，给予大蒜可预防氧化应激，恢复铅（Pb(2+)）暴露小鼠红细胞中的抗氧化平衡。此外，体外研究表明，在铅（Pb(2+)）暴露期间，在二硫苏糖醇存在下修饰关键巯基可改变磷脂酰丝氨酸转位酶和氨基磷脂转位酶的活性。数据还表明，铅（Pb(2+)）暴露小鼠经大蒜治疗后，磷脂酰丝氨酸暴露急剧下降，红细胞膜巯基增加，随后氨基磷脂转位酶活性增加，磷脂酰丝氨酸转位酶活性下降。研究结果表明，大蒜具有在铅（Pb(2+)）暴露期间恢复红细胞寿命的能力。
