CEA LETI MINATEC, Department of Technology for Biology and Healthcare, 17 avenue des Martyrs, Grenoble, F-38054, France.
Phys Chem Chem Phys. 2013 Oct 14;15(38):15840-4. doi: 10.1039/c3cp51281a. Epub 2013 Sep 2.
A low-cost, innovative and non-invasive colorimetric test, which can be universally used, is proposed to detect pathogenic bacteria via the simple and fast detection of volatile metabolites released by enzymatic hydrolysis. The proof of concept is shown via three sets of experiments studying the release of the p-nitrophenol metabolite in solution in the E. coli cultures containing 4-nitrophenyl-β-d-glucuronide, the trapping efficiency of the gaseous metabolite by various tailored and functionalized xerogels, and the trapping and detection of gaseous p-nitrophenol released by E. coli bacteria.
提出了一种低成本、创新且非侵入性的比色测试方法，可通过简单快速地检测酶解释放的挥发性代谢物来检测病原菌。通过三组实验证明了这一概念，这些实验研究了含有 4-硝基苯-β-d-葡糖苷酸的大肠杆菌培养物中 p-硝基苯酚代谢物在溶液中的释放、各种定制和功能化干凝胶对气态代谢物的捕集效率，以及大肠杆菌细菌释放的气态 p-硝基苯酚的捕集和检测。
