Department of Pathology and Wildlife Diseases, National Veterinary Institute, Uppsala, Sweden.
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Epidemiol Infect. 2014 Jun;142(6):1205-13. doi: 10.1017/S0950268813002094. Epub 2013 Sep 4.
The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n = 263), and for Anaplasma antibodies with ELISA serology (n = 234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P < 0·01) temporal, and spatial variation was found. Island moose had significantly (P < 0·001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen.
在瑞典南部（岛屿和大陆）的瑞典驼鹿（Alces alces）的脾脏和血清样本中，研究了吞噬细胞无形体（Anaplasma phagocytophilum）的发生情况。通过实时 PCR（n = 263）分析样本中是否存在 A. phagocytophilum DNA，并通过 ELISA 血清学（n = 234）分析是否存在抗 A. phagocytophilum 抗体。所有血清样本均具有针对 A. phagocytophilum 的抗体。基于 DNA 的患病率平均为 26.3%，并发现了明显的（P < 0.01）时间和空间变化。与来自大陆地区的驼鹿相比，岛屿驼鹿的 A. phagocytophilum DNA 患病率明显（P < 0.001）更高。对两个样本进行测序以确定 16S rRNA 和 groESL 基因中的遗传变异。在 16S rRNA 和 groESL 基因中观察到与人类粒细胞无形体病病原体、马粒细胞埃立克体病病原体以及不同野生动物相关的 A. phagocytophilum 变体的遗传序列相似性。我们的研究表明，瑞典的驼鹿暴露于 A. phagocytophilum 之下，代表了病原体的潜在野生动物储存库。
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