Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7 ET, United Kingdom.
Free Radic Biol Med. 2013 Dec;65:925-941. doi: 10.1016/j.freeradbiomed.2013.08.184. Epub 2013 Aug 31.
In this review, we provide a comprehensive bibliographic overview of the role of mass spectrometry and the recent technical developments in the detection of post-translational modifications (PTMs). We briefly describe the principles of mass spectrometry for detecting PTMs and the protein and peptide enrichment strategies for PTM analysis, including phosphorylation, acetylation and oxidation. This review presents a bibliographic overview of the scientific achievements and the recent technical development in the detection of PTMs is provided. In order to ascertain the state of the art in mass spectrometry and proteomics methodologies for the study of PTMs, we analyzed all the PTM data introduced in the Universal Protein Resource (UniProt) and the literature published in the last three years. The evolution of curated data in UniProt for proteins annotated as being post-translationally modified is also analyzed. Additionally, we have undertaken a careful analysis of the research articles published in the years 2010 to 2012 reporting the detection of PTMs in biological samples by mass spectrometry.
在这篇综述中，我们提供了一个全面的文献综述，介绍了质谱在检测翻译后修饰（PTMs）中的作用和最近的技术发展。我们简要描述了用于检测 PTM 的质谱原理以及用于 PTM 分析的蛋白质和肽富集策略，包括磷酸化、乙酰化和氧化。本综述提供了一个文献综述，介绍了检测 PTM 的科学成就和最近的技术发展。为了确定用于研究 PTM 的质谱和蛋白质组学方法的最新技术，我们分析了在通用蛋白质资源（UniProt）中引入的所有 PTM 数据以及在过去三年中发表的文献。还分析了 UniProt 中注释为翻译后修饰的蛋白质的经过精心整理的数据的演变。此外，我们还对 2010 年至 2012 年期间发表的报道通过质谱在生物样本中检测 PTM 的研究文章进行了仔细分析。
