Huang Junfeng, Wang Fangjun, Ye Mingliang, Zou Hanfa
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; University of Chinese Academy of Sciences, Beijing, China.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
J Chromatogr A. 2014 Dec 12;1372C:1-17. doi: 10.1016/j.chroma.2014.10.107. Epub 2014 Nov 6.
Comprehensive analysis of the post-translational modifications (PTMs) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. In the past decades, thanks to the development of specific PTM enrichment techniques and efficient multidimensional liquid chromatography (LC) separation strategy, the identification of protein PTMs have made tremendous progress. A huge number of modification sites for some major protein PTMs have been identified by proteomics analysis. In this review, we first introduced the recent progresses of PTM enrichment methods for the analysis of several major PTMs including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. We then briefly summarized the challenges for PTM enrichment. Finally, we introduced the fractionation and separation techniques for efficient separation of PTM peptides in large-scale PTM analysis.
在蛋白质组水平上对蛋白质的翻译后修饰（PTM）进行全面分析，对于阐明各种生物过程的调控机制至关重要。在过去几十年中，由于特定PTM富集技术和高效多维液相色谱（LC）分离策略的发展，蛋白质PTM的鉴定取得了巨大进展。通过蛋白质组学分析已经鉴定出了一些主要蛋白质PTM的大量修饰位点。在本综述中，我们首先介绍了用于分析几种主要PTM（包括磷酸化、糖基化、泛素化、乙酰化、甲基化和氧化/还原状态）的PTM富集方法的最新进展。然后，我们简要总结了PTM富集面临的挑战。最后，我们介绍了在大规模PTM分析中有效分离PTM肽段的分级分离和分离技术。
