Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Chongqing, 400016, People's Republic of China.
Med Oncol. 2013 Dec;30(4):613. doi: 10.1007/s12032-013-0613-9. Epub 2013 Sep 5.
The ubiquitin-like with PHD and ring finger domains 1 (UHRF1) has been reported to be essential for cell proliferation and plays a critical role in the development and progression of many human carcinomas. However, its clinical and prognostic significance in laryngeal squamous cell carcinoma (LSCC) remains unclear. In the current study, 60 patients with LSCC were studied. UHRF1 expression at mRNA and protein levels was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunohistochemistry, respectively, in both tissues of LSCC and corresponding adjacent normal larynx tissues. Statistical analyses were conducted to test the correlations between UHRF1 expression, clinicopathological parameters, and prognosis. qRT-PCR showed that the expression of UHRF1 mRNA in LSCC tissues was significantly higher than that in adjacent normal larynx tissues (P < 0.001). By immunohistochemistry, overexpression of UHRF1 protein was found in 78.3% (47/60) of the LSCC tissues, while there was negative expression in adjacent normal larynx tissues. Furthermore, increased expression of UHRF1 had remarkably positive relationships with smoking (P < 0.001), advanced T stage (P = 0.005) and clinical stage (P = 0.044), poor histological differentiation (P = 0.048), while there was no correlations between UHRF1, and sex, age and lymph node metastasis (P > 0.05). Overexpression of UHRF1 was also associated with worse overall survival examined by Kaplan-Meier method (P = 0.036). Multivariate analysis showed that increased expression of UHRF1 was an independent prognostic factor for LSCC (P = 0.013). Therefore, overexpression of UHRF1 may play an important role in the development and progression of LSCC, and UHRF1 might be a useful biomarker for the prognosis of LSCC.
泛素样含 PH 和环指结构域蛋白 1（UHRF1）已被报道对细胞增殖至关重要，并在许多人类癌的发生和发展中发挥关键作用。然而，其在喉鳞状细胞癌（LSCC）中的临床和预后意义尚不清楚。在本研究中，研究了 60 例 LSCC 患者。通过定量逆转录聚合酶链反应（qRT-PCR）和免疫组织化学分别检测 LSCC 组织和相应的相邻正常喉组织中 UHRF1 的 mRNA 和蛋白水平表达。进行统计学分析以检验 UHRF1 表达与临床病理参数和预后之间的相关性。qRT-PCR 显示 LSCC 组织中 UHRF1 mRNA 的表达明显高于相邻正常喉组织（P <0.001）。通过免疫组织化学，发现 78.3%（47/60）的 LSCC 组织中 UHRF1 蛋白呈过表达，而相邻正常喉组织呈阴性表达。此外，UHRF1 表达增加与吸烟（P <0.001）、晚期 T 分期（P = 0.005）和临床分期（P = 0.044）、组织学分化不良（P = 0.048）显著正相关，而与 UHRF1 与性别、年龄和淋巴结转移无相关性（P >0.05）。Kaplan-Meier 法检查发现 UHRF1 过表达与总生存率降低有关（P = 0.036）。多变量分析显示，UHRF1 表达增加是 LSCC 的独立预后因素（P = 0.013）。因此，UHRF1 的过表达可能在 LSCC 的发生和发展中起重要作用，UHRF1 可能是 LSCC 预后的有用标志物。
