Gene Regulation Research, Nara Institute Science and Technology, 8916-5 Takayama, Nara 630-0101, Japan.
Int J Mol Sci. 2013 Sep 3;14(9):18009-23. doi: 10.3390/ijms140918009.
We recently reported that an RNA binding protein called Cugbp Elav-like family member 1 (Celf1) regulates somite symmetry and left-right patterning in zebrafish. In this report, we show additional roles of Celf1 in zebrafish organogenesis. When celf1 is knocked down by using an antisense morpholino oligonucleotides (MO), liver buds fail to form, and pancreas buds do not form a cluster, suggesting earlier defects in endoderm organogenesis. As expected, we found failures in endoderm cell growth and migration during gastrulation in embryos injected with celf1-MOs. RNA immunoprecipitation revealed that Celf1 binds to gata5 and cdc42 mRNAs which are known to be involved in cell growth and migration, respectively. Our results therefore suggest that Celf1 regulates proper organogenesis of endoderm-derived tissues by regulating the expression of such targets.
我们最近报道了一种称为 Cugbp Elav-like family member 1（Celf1）的 RNA 结合蛋白，它在斑马鱼中调节体节对称性和左右模式。在本报告中，我们展示了 Celf1 在斑马鱼器官发生中的其他作用。当使用反义的 morpholino 寡核苷酸（MO）敲低 celf1 时，肝芽不能形成，胰腺芽不能形成簇，这表明内胚层器官发生早期存在缺陷。正如预期的那样，我们发现注射 celf1-MO 的胚胎在原肠胚形成期间内胚层细胞的生长和迁移出现了失败。RNA 免疫沉淀显示 Celf1 与 gata5 和 cdc42 mRNA 结合，这两种 mRNA 分别已知参与细胞生长和迁移。因此，我们的结果表明，Celf1 通过调节这些靶标基因的表达来调节内胚层衍生组织的正常发生。
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