Kennedy S H, Sargent I L, Starkey P M, Hicks B R, Barlow D H
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford.
Br J Obstet Gynaecol. 1990 Aug;97(8):671-4. doi: 10.1111/j.1471-0528.1990.tb16236.x.
Anti-endometrial antibody binding was localized using a double-labelling immunohistochemical method on frozen sections of endometrium taken from a woman without pelvic disease. Serum from 40 women with endometriosis was tested and, as controls, serum samples from 20 adult males and 20 umbilical cords. The method allowed compensation for endogenous immunoglobulins in endometrium and accurate localization of anti-endometrial antibody binding in the cytoplasm of the glandular epithelium. Significantly more women with endometriosis (14/40) were found to have anti-endometrial antibodies than controls (1/40) (P less than 0.001; chi 2). There was no correlation between disease severity and the presence of anti-endometrial antibodies or the intensity of staining.
采用双标记免疫组化方法，对取自一名无盆腔疾病女性的子宫内膜冰冻切片进行抗子宫内膜抗体结合定位。检测了40例子宫内膜异位症女性的血清，并作为对照，检测了20名成年男性和20份脐带血清样本。该方法可补偿子宫内膜中的内源性免疫球蛋白，并能准确将抗子宫内膜抗体结合定位在腺上皮细胞质中。发现子宫内膜异位症女性中有抗子宫内膜抗体的人数（14/40）明显多于对照组（1/40）（P<0.001；卡方检验）。疾病严重程度与抗子宫内膜抗体的存在或染色强度之间无相关性。
