Fernández-Shaw S, Hicks B R, Yudkin P L, Kennedy S, Barlow D H, Starkey P M
University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, UK.
Hum Reprod. 1993 Feb;8(2):310-5. doi: 10.1093/oxfordjournals.humrep.a138042.
The present study investigates the specificity of anti-endometrial antibodies present in serum of women with endometriosis. A two colour indirect immuno-histochemical method was used, so that the antigenic reactivity of endogenous immunoglobulins was blocked and specific anti-endometrial antibodies were readily distinguishable. Serum was collected from women with endometriosis, before and after 6 months of treatment, and from women without the disease. The reactivity of serum with uterine and ectopic endometrium from women with and without the disease was studied. The frequency of anti-endometrial antibodies in the serum of women with endometriosis was higher (P < 0.001) than in control sera. Most antibodies specifically reacted with glands in ectopic and uterine endometrium. Antibody reactivity was strongest with endometrium from control women, compared with uterine and ectopic endometrium from women with endometriosis (P < 0.01). A proportion of sera containing anti-endometrial antibodies also reacted with vascular endothelium. Binding was strongest to vessels in uterine and ectopic endometrium from women with endometriosis compared to endometrium from women without the disease (P < 0.01). The presence of anti-endometrial antibodies was associated with infertility.
本研究调查了子宫内膜异位症女性血清中抗子宫内膜抗体的特异性。采用双色间接免疫组织化学方法，从而阻断内源性免疫球蛋白的抗原反应性，并使特异性抗子宫内膜抗体易于区分。收集了子宫内膜异位症女性治疗前、治疗6个月后的血清以及未患该病女性的血清。研究了这些血清与患病人群和未患病人群的子宫及异位子宫内膜的反应性。子宫内膜异位症女性血清中抗子宫内膜抗体的频率高于对照血清（P<0.001）。大多数抗体与异位和子宫子宫内膜中的腺体发生特异性反应。与子宫内膜异位症女性的子宫和异位子宫内膜相比，抗体与对照女性子宫内膜的反应性最强（P<0.01）。一部分含有抗子宫内膜抗体的血清也与血管内皮发生反应。与未患该病女性的子宫内膜相比，与子宫内膜异位症女性的子宫和异位子宫内膜中的血管结合最强（P<0.01）。抗子宫内膜抗体的存在与不孕有关。
