Plant Tissue Culture Laboratory, Department of Horticulture, Pir Mehr Ali Shah-Arid Agriculture University, Rawalpindi 46300, Pakistan.
J Zhejiang Univ Sci B. 2013 Sep;14(9):774-84. doi: 10.1631/jzus.B1200277.
In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.
采用 10%次氯酸钠对顶端芽进行表面消毒，可获得 70%的最大存活率。采用不同浓度的细胞分裂素苄氨基嘌呤（BAP）、激动素和 N6-（2-异戊基）腺嘌呤（2iP）组合，在 Murashige 和 Skoog（MS）培养基上评估愈伤组织发生反应。BAP 1.5 mg/L 与 2iP 9.0 mg/L 和激动素 1.5 mg/L 的处理在芽形态发生的芽数量和随后的生长方面被发现是最佳的，而 BAP 0.5 mg/L、激动素 0.5 mg/L 和 2iP 3 mg/L 的组合产生了最高的芽长度。较高水平的细胞分裂素在某种程度上诱导了愈伤组织发生、玻璃化和生长不良。对于生根，将大小均匀的微型芽切下并转移到含有生长素的半强度 MS 培养基中。萘乙酸（NAA）1 mg/L 与吲哚丁酸（IBA）2 mg/L 和多效唑（PBZ）1 mg/L 联合使用时，观察到最佳的生根表达。
