Wiesman Z, Riov J, Epstein E
Department of Horticulture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Plant Physiol. 1989 Nov;91(3):1080-4. doi: 10.1104/pp.91.3.1080.
Indole-3-butyric acid (IBA) is rapidly metabolized by mung bean cuttings during rooting. Twenty-four hours after application, less than 20% of the applied IBA remained in the free form and its level decreased continuously in the later stages of rooting. Indole-3-butyrylaspartic acid (IBAsp) and at least two high molecular weight conjugates were the major metabolites in IBA-treated cuttings. In the latter conjugates, at least part of the IBA moiety is attached to a high molecular weight constituent in an amide linkage. IBAsp level peaked 24 hours after application of IBA to the cuttings and then declined. The level of the high molecular weight conjugates increased continuously throughout the rooting process. The conjugates were active in inducing rooting of cuttings, with IBAsp being superior to free IBA. It is suggested that IBA conjugates, and particularly IBAsp, serve as the source of auxin during the later stages of rooting.
吲哚 - 3 - 丁酸（IBA）在绿豆插条生根过程中会迅速被代谢。施用后24小时，施用的IBA中不到20%以游离形式存在，且在生根后期其水平持续下降。吲哚 - 3 - 丁酰天冬氨酸（IBAsp）和至少两种高分子量结合物是IBA处理插条中的主要代谢产物。在后者的结合物中，至少部分IBA部分通过酰胺键与高分子量成分相连。IBAsp水平在向插条施用IBA后24小时达到峰值，然后下降。高分子量结合物的水平在整个生根过程中持续增加。这些结合物在诱导插条生根方面具有活性，其中IBAsp优于游离IBA。有人认为，IBA结合物，特别是IBAsp，在生根后期作为生长素的来源。
