Pompeiano M, Palacios J M, Mengod G
Sandoz Pharma Limited, Basel, Switzerland.
J Neurosci. 1992 Feb;12(2):440-53. doi: 10.1523/JNEUROSCI.12-02-00440.1992.
In order to localize the cells expressing 5-HT1A receptors in the rat brain, we used in situ hybridization histochemistry to visualize the distribution of the mRNA coding for 5-HT1A receptors. Oligonucleotides derived from different parts of the coding region of the rat 5-HT1A receptor gene were used as hybridization probes. 5-HT1A binding sites were visualized on consecutive sections by receptor autoradiography using 3H-8-hydroxy-2-(di-n-propylamino)tetralin as ligand. The highest levels of hybridization were observed in the dorsal raphe nucleus, septum, hippocampus, entorhinal cortex, and interpeduncular nucleus. Positive hybridization signals were also present in other areas, such as the olfactory bulb; cerebral cortex; some thalamic and hypothalamic nuclei; several nuclei of the brainstem, including all the remaining raphe nuclei, nucleus of the solitary tract, and nucleus of the spinal tract of the trigeminus; and the dorsal horn of the spinal cord. The distribution and abundance of 5-HT1A receptor mRNA in different rat brain areas generally correlate with those of the binding sites, suggesting that 5-HT1A receptors are predominantly somatodendritic receptors.
为了定位大鼠脑中表达5-HT1A受体的细胞，我们采用原位杂交组织化学方法来观察编码5-HT1A受体的mRNA的分布情况。从大鼠5-HT1A受体基因编码区不同部位获得的寡核苷酸被用作杂交探针。使用3H-8-羟基-2-（二正丙基氨基）四氢萘作为配体，通过受体放射自显影术在连续切片上观察5-HT1A结合位点。在中缝背核、隔区、海马、内嗅皮质和脚间核中观察到最高水平的杂交信号。阳性杂交信号也出现在其他区域，如嗅球、大脑皮质、一些丘脑和下丘脑核团、脑干的几个核团（包括所有其余的中缝核、孤束核和三叉神经脊束核）以及脊髓背角。5-HT1A受体mRNA在大鼠不同脑区的分布和丰度通常与结合位点的分布和丰度相关，这表明5-HT1A受体主要是树突-胞体受体。
