Kono K, Tsuchida T, Kern D H, Irie R
Division of Surgical Oncology, John Wayne Clinic, Armand Hammer Laboratories, Jonsson Comprehensive Cancer Center, UCLA School of Medicine 90024.
Cancer Invest. 1990;8(2):161-7. doi: 10.3109/07357909009017561.
Ganglioside composition of human melanoma was correlated with sensitivity of melanoma to antitumor treatment with chemotherapeutic agents and radiation. The cytotoxic effect of each treatment was evaluated on 16 melanoma cell lines using the human tumor colony-forming assay. Ganglioside fractions were extracted and purified from each cell line and analyzed for the four major gangliosides in melanoma (GM3, GM2, GD3, and GD2) by thin-layer chromatography (TLC) and TLC scanner. GD2 content positively correlated with sensitivity to radiation (r = 0.753, p less than 0.001) and vincristine (r = 0.779, p less than 0.001). In contrast, GM3 content inversely correlated with sensitivity to radiation (r = -0.658, p less than 0.01) and vincristine (r = -0.692, less than 0.01). The gangliosides GD3 and GM2 were shown to have no significant correlation with any of these treatments.
人类黑色素瘤的神经节苷脂组成与黑色素瘤对化疗药物和放疗的抗肿瘤治疗敏感性相关。使用人类肿瘤集落形成试验评估了每种治疗对16种黑色素瘤细胞系的细胞毒性作用。从每个细胞系中提取并纯化神经节苷脂组分，并通过薄层色谱法（TLC）和TLC扫描仪分析黑色素瘤中的四种主要神经节苷脂（GM3、GM2、GD3和GD2）。GD2含量与对放疗的敏感性呈正相关（r = 0.753，p小于0.001）和长春新碱（r = 0.779，p小于0.001）。相反，GM3含量与对放疗的敏感性呈负相关（r = -0.658，p小于0.01）和长春新碱（r = -0.692，小于0.01）。已表明神经节苷脂GD3和GM2与这些治疗中的任何一种均无显著相关性。
