Hong Yan, Han Yan-Quan, Xia Lun-Zhu, Gui Jie, Chen Xi, Sun Yan-Hua
School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230031, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1766-71.
This study was establish an UPLC fingerprint of Xanthii Fructus from different habitats, to provide a comprehensive evaluation for its quality control. UPLC-PDA was adopted to analysis of 26 baches of Xanthii Fructus from different habitats. The chromatographic condition was as follow: ACQUITY BEH C18 Column (2.1 mm x 100 mm,1.7 microm) eluted with the mobile phases of acetonitrile and 0.1% phosphoric acid water in gradient mode. The flow rate was 0.25 mL x min(-1) and the detection wavelength was set at 220 nm. The fingerprints of 26 batches Xanthii Fructus were carried out by similarity comparation, cluster and the principal component analysis (PCA). There were nineteen common peaks, nine of which had been identified, and the similarity degrees of the twenty-six batches of the samples were between 0.804 and 0.990. All the samples were classified into six categories, and the PCA value of each fingerprint peak was calculated, and six principal components accounted for over 81. 140% of the total variance were extracted from the original data This method can be used to assess the quality of Xanthii Fructus.
本研究旨在建立不同产地苍耳子的超高效液相色谱指纹图谱，为其质量控制提供全面评价。采用超高效液相色谱-二极管阵列检测器（UPLC-PDA）对26批不同产地的苍耳子进行分析。色谱条件如下：ACQUITY BEH C18柱（2.1 mm×100 mm，1.7 µm），以乙腈和0.1%磷酸水溶液为流动相进行梯度洗脱。流速为0.25 mL·min-1，检测波长设定为220 nm。对26批苍耳子的指纹图谱进行相似度比较、聚类和主成分分析（PCA）。共有19个共有峰，其中9个已被鉴定，26批样品的相似度在0.804至0.990之间。所有样品被分为6类，并计算每个指纹峰的PCA值，从原始数据中提取出占总方差81.140%以上的6个主成分。该方法可用于评价苍耳子的质量。
