Walker Sarah E, Lorsch Jon
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Enzymol. 2013;529:171-84. doi: 10.1016/B978-0-12-418687-3.00014-8.
While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.
虽然自动化DNA测序的便捷性和成本降低在很大程度上消除了常规用途中对手动双脱氧测序的需求，但特定应用仍需要手动DNA测序。例如，在研究与DNA结合或修饰DNA的酶或蛋白质时，通常使用DNA梯来定位酶结合或发生修饰的位点。在这些情况下，双脱氧测序的桑格法提供了一种快速简便的方法来生成标记的DNA梯。
