Furudate S, Nakano T
Department of Laboratory Animal Science, Kitasato University School of Medicine, Kanagawa, Japan.
Jikken Dobutsu. 1990 Jul;39(3):337-43. doi: 10.1538/expanim1978.39.3_337.
Urethane-anesthetized male rats have been used for the analysis of prolactin (PRL)-releasing substances on PRL secretion. However, there are only a few reports investigating the effect of urethane anesthesia on PRL secretion in female rats. In this study, we intended to examine the effects of urethane anesthesia on PRL secretion during proestrus in the rat. Proestrus PRL surge was completely blocked when urethane was administered to rats prior to the critical period of proestrus both at doses of 1.0 g/kg and 1.5 g/kg. Additionally, urethane, at a dose of 1.5 g/kg, was also effective in blocking spontaneous ovulation. An experiment examining pituitary PRL concentration at 1800 h confirmed that urethane (1.0 g/kg) anesthesia prevents the PRL surge from the pituitary. Similarly, urethane anesthesia blocked the LH surge from the pituitary, but LH levels in the urethane-treated group were higher than those in the pentobarbital-treated group.
氨基甲酸乙酯麻醉的雄性大鼠已被用于分析催乳素（PRL）释放物质对PRL分泌的影响。然而，仅有少数报告研究氨基甲酸乙酯麻醉对雌性大鼠PRL分泌的影响。在本研究中，我们旨在检测氨基甲酸乙酯麻醉对大鼠发情前期PRL分泌的影响。当在发情前期的关键时期之前给大鼠注射剂量为1.0 g/kg和1.5 g/kg的氨基甲酸乙酯时，发情前期PRL高峰被完全阻断。此外，剂量为1.5 g/kg的氨基甲酸乙酯在阻断自发排卵方面也有效。一项在18:00检测垂体PRL浓度的实验证实，氨基甲酸乙酯（1.0 g/kg）麻醉可阻止垂体释放PRL高峰。同样，氨基甲酸乙酯麻醉阻断了垂体释放LH高峰，但氨基甲酸乙酯处理组的LH水平高于戊巴比妥处理组。
