Dong Ya-Chen, Dai Yi-Ning, Xu Teng-Yang, Cai Jin, Chen Qi-He
Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China.
Bioprocess Biosyst Eng. 2014 May;37(5):755-64. doi: 10.1007/s00449-013-1045-9. Epub 2013 Sep 8.
As a discarded lignocellulosic biomass, chestnut shell is of great potential economic value, thus a sustainable strategy is needed and valuable for utilization of this resource. Herein, the feasibility of biological processes of chestnut shell with Dichomitus squalens, Phlebia radiata and their co-cultivation for lignin-modifying enzymes (LMEs) production and biodegradation of this lignocellulosic biomass was investigated under submerged cultivation. The treatment with D. squalens alone at 12 days gained the highest laccase activity (9.42 ± 0.73 U mg(-1)). Combined with the data of laccase and manganese peroxidase, oxalate and H2O2 were found to participate in chestnut shell degradation, accompanied by a rapid consumption of reducing sugar. Furthermore, specific surface area of chestnut shell was increased by 77.6-114.1 % with the selected fungi, and total pore volume was improved by 90.2 % with D. squalens. Meanwhile, the surface morphology was observably modified by this fungus. Overall, D. squalens was considered as a suitable fungus for degradation of chestnut shell and laccase production. The presence of LMEs, H2O2 and oxalate provided more understanding for decomposition of chestnut shell by the white-rot fungi.
作为一种废弃的木质纤维素生物质，栗壳具有巨大的潜在经济价值，因此需要一种可持续的策略来利用这种资源，这具有重要意义。在此，研究了在 submerged cultivation 条件下，利用 squalens 栓菌、辐射脉菌及其共培养生物处理栗壳以生产木质素修饰酶（LMEs）和对这种木质纤维素生物质进行生物降解的可行性。单独用 squalens 栓菌处理 12 天时获得了最高的漆酶活性（9.42±0.73 U mg(-1)）。结合漆酶和锰过氧化物酶的数据发现，草酸盐和过氧化氢参与了栗壳的降解，同时伴随着还原糖的快速消耗。此外，所选真菌使栗壳的比表面积增加了 77.6 - 114.1%，用 squalens 栓菌处理后总孔体积提高了 90.2%。同时，这种真菌显著改变了栗壳的表面形态。总体而言，squalens 栓菌被认为是一种适合降解栗壳和生产漆酶的真菌。LMEs、过氧化氢和草酸盐的存在为白腐真菌分解栗壳提供了更多的认识。
