Gardner D P, Shimizu N
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
FEBS Lett. 1990 Sep 3;269(2):288-91. doi: 10.1016/0014-5793(90)81178-q.
Epidermal growth factor receptor gene expression in response to the tumor promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was analyzed in the A549 human lung adenocarcinoma cell line using DNA transfection. After 8 h of treatment a definite repression (45%) of EGF receptor gene transcription could be seen. Repression was maintained for up to 24 h. Little or no induction of transcription could be seen prior to the onset of repression for potential enhancer regions both upstream and downstream of the translation start site. The cis-element(s) responsible for repression were localized between residues -384 and -151 of the EGF receptor gene promoter region using a deletion plasmid series.
使用DNA转染技术，在A549人肺腺癌细胞系中分析了表皮生长因子受体基因对促肿瘤佛波酯12 - O - 十四烷酰佛波醇-13 - 乙酸酯（TPA）的表达响应。处理8小时后，可观察到表皮生长因子受体基因转录明显受到抑制（45%）。这种抑制作用持续长达24小时。在翻译起始位点上游和下游的潜在增强子区域的抑制开始之前，几乎看不到转录的诱导。使用一系列缺失质粒，将负责抑制的顺式元件定位在表皮生长因子受体基因启动子区域的-384至-151位残基之间。
