Garrett L R, Jones C J, Cuchens M A
Department of Microbiology University of Mississippi Medical Center, Jackson 39216-4505.
Chem Biol Interact. 1992 Jan;81(1-2):119-30. doi: 10.1016/0009-2797(92)90030-o.
Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.
开展了多项研究，以检测2,6,10,14 - 四甲基十五烷（降植烷）与12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）对转染至NIH - 3T3、CV - 1和COS - 7细胞中的病毒启动子/增强子元件调控下的CAT基因激活的影响。这些研究结果表明：（1）在用各自相应质粒构建体转染的细胞中，降植烷或TPA诱导SV2cat、HIVcat、RSVcat和MMTVcat的反式激活；（2）仅降植烷诱导pA10cat和pOSP/11的激活；（3）TPA和降植烷均未反式激活pSV0cat。此外，用降植烷或TPA处理均引起各细胞系形态的变化。总体而言，这些结果表明降植烷是一种有效的基因表达诱导剂，并且表现出与肿瘤启动子TPA相似的特征。
