Kato I, Yonekura H, Yamamoto H, Okamoto H
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
FEBS Lett. 1990 Sep 3;269(2):319-23. doi: 10.1016/0014-5793(90)81184-p.
We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
我们证明，在大鼠垂体中，肽基甘氨酸α-酰胺化酶由至少5种不同的mRNA编码。Southern印迹和核糖核酸酶保护分析表明，这些mRNA是通过可变剪接产生的。一种缺乏跨膜结构域编码序列的变体是垂体中该酶的主要mRNA种类。当这些cDNA在COS-7细胞中表达时，该变体在产生该酶的分泌形式（37 kDa）方面效率最高。
