Glauder J, Ragg H, Rauch J, Engels J W
Universität Frankfurt, Institut für Organische Chemie, FRG.
Biochem Biophys Res Commun. 1990 Jun 15;169(2):551-8. doi: 10.1016/0006-291x(90)90366-u.
We have identified a cDNA encoding human peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) with a total length of 3748 bp by screening of a human thyroid carcinoma lambda gt11 library using two heterologous oligonucleotides to conserved regions which derived from frog skin and bovine pituitary PAM sequences. Furthermore we have identified a sequence which differs in a 321 bp deletion. COS cells transfected with a truncated form of this cDNA (lacking the putative carboxyl-terminal transmembrane domain) generated a functional PAM that showed a 20-fold increase of the activity compared to the control and was visualized by immunoblotting.
我们通过使用两个源自蛙皮和牛垂体PAM序列保守区域的异源寡核苷酸筛选人甲状腺癌λgt11文库，鉴定出了一个全长3748 bp的编码人肽基甘氨酸α-酰胺化单加氧酶（PAM；EC 1.14.17.3）的cDNA。此外，我们还鉴定出了一个在321 bp缺失上存在差异的序列。用该cDNA的截短形式（缺少假定的羧基末端跨膜结构域）转染的COS细胞产生了一种功能性PAM，与对照相比，其活性增加了20倍，并通过免疫印迹法得以可视化。
