Optical-Bio Microsystem Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, QC, Canada, H3G 1M8.
Biomed Microdevices. 2014 Feb;16(1):23-33. doi: 10.1007/s10544-013-9802-8.
A biocompatible polydimethylsiloxane (PDMS) biomicrofluidic platform is designed, fabricated and tested to study protuberance growth of single plant cells in a micro-vitro environment. The design consists of an inlet to introduce the cell suspension into the chip, three outlets to conduct the medium or cells out of the chip, a main distribution chamber and eight microchannels connected to the main chamber to guide the growth of tip growing plant cells. The test cells used here were pollen grains which produce cylindrical protrusions called pollen tubes. The goal was to adjust the design of the microfluidic network with the aim to enhance the uniformly distributed positioning of pollen grains at the entrances of the microchannels and to provide identical fluid flow conditions for growing pollen tubes along each microchannel. Computational fluid analysis and experimental testing were carried out to estimate the trapping efficiencies of the different designs.
设计、制造和测试了一种生物相容性的聚二甲基硅氧烷（PDMS）生物微流控平台，以研究在微环境中单个植物细胞的突起生长。该设计包括一个入口，用于将细胞悬浮液引入芯片；三个出口，用于将介质或细胞从芯片中导出；一个主分配室和八个微通道，与主室相连，用于引导顶端生长的植物细胞的生长。这里使用的测试细胞是花粉粒，它产生称为花粉管的圆柱形突起。目标是调整微流控网络的设计，以增强花粉粒在微通道入口处的均匀分布定位，并为每个微通道中生长的花粉管提供相同的流体流动条件。进行了计算流体分析和实验测试，以估计不同设计的捕获效率。
