Schuren F H, Wessels J G
Department of Plant Biology, University of Groningen, Haren, The Netherlands.
Gene. 1990 Jun 15;90(2):199-205. doi: 10.1016/0378-1119(90)90180-y.
The nucleotide (nt) sequences of the Sc3 and Sc4 genes of the filamentous fungus Schizophyllum commune, and the deduced amino acid (aa) sequences, were determined; moreover, the previously published sequence for the Sc1 gene [Dons et al., EMBO J. 3 (1984) 2101-2106] was corrected. All three independently isolated genes were found to have similar structures and nt sequences of their coding regions. At the aa level the homology is 43-62% (63-69% in the C-terminal parts of the proteins), the hydrophobic aa predominate and the hydrophobicity patterns are similar. All three proteins contain leader sequences and eight cysteines among about 110 aa, conserved at the same positions. Yet these genes are differentially regulated: Sc1 and Sc4 are only expressed at high levels in fruiting dikaryons, whereas Sc3 is highly expressed in both monokaryons and dikaryons, independent from fruiting.
测定了丝状真菌裂褶菌（Schizophyllum commune）Sc3和Sc4基因的核苷酸（nt）序列以及推导的氨基酸（aa）序列；此外，对先前发表的Sc1基因序列[Dons等人，《欧洲分子生物学组织杂志》3（1984年）2101 - 2106]进行了校正。发现所有三个独立分离的基因具有相似的结构及其编码区的nt序列。在氨基酸水平上，同源性为43 - 62%（蛋白质C端部分为63 - 69%），疏水氨基酸占主导且疏水模式相似。所有三种蛋白质都含有前导序列，在约110个氨基酸中含有八个半胱氨酸，且在相同位置保守。然而，这些基因受到不同的调控：Sc1和Sc4仅在结实双核体中高水平表达，而Sc3在单核体和双核体中均高水平表达，与结实无关。
