Schuren F H, Asgeirsdóttir S A, Kothe E M, Scheer J M, Wessels J G
Department of Plant Biology, University of Groningen, Haren, The Netherlands.
J Gen Microbiol. 1993 Sep;139(9):2083-90. doi: 10.1099/00221287-139-9-2083.
The Sc7 and Sc14 genes are specifically expressed in the dikaryon of the basidiomycete fungus Schizophyllum commune during fruiting. These genes are closely linked (within 6 kb) and highly similar in gene structure and nucleotide sequence (70% identical nucleotides in their coding regions). The encoded proteins (204 and 214 amino acids, respectively) have 87% similarity in amino acids (56% of the amino acids are identical). They contain putative signal sequences for secretion, are rich in aromatic amino acids which are generally located at similar positions, and they are generally hydrophilic. Inspection of databanks showed similarities with pathogenesis-related proteins (PR1) from plants, testis-specific proteins from mammals and venom allergen proteins from insects. An antibody raised against a Sc7 fusion protein showed the presence of the Sc7 protein in the culture medium and in the fruit bodies where it is apparently loosely associated with hyphal walls.
Sc7和Sc14基因在担子菌裂褶菌结实期间的双核体中特异性表达。这些基因紧密连锁（在6 kb范围内），基因结构和核苷酸序列高度相似（编码区70%的核苷酸相同）。编码的蛋白质（分别为204和214个氨基酸）氨基酸相似性为87%（56%的氨基酸相同）。它们含有推测的分泌信号序列，富含芳香族氨基酸，这些氨基酸通常位于相似位置，且总体呈亲水性。数据库检索显示，它们与植物的病程相关蛋白（PR1）、哺乳动物的睾丸特异性蛋白以及昆虫的毒液过敏原蛋白相似。针对Sc7融合蛋白产生的抗体表明，Sc7蛋白存在于培养基和子实体中，在子实体中它显然与菌丝壁松散结合。
