Froeliger E H, Ullrich R C, Novotny C P
Department of Microbiology, University of Vermont, Burlington 05405.
Gene. 1989 Nov 30;83(2):387-93. doi: 10.1016/0378-1119(89)90127-3.
The URA1 gene (encoding orotidine-5'-monophosphate decarboxylase) of the basidiomycete fungus Schizophyllum commune was mapped to a 1.4-kb BglI-BamHI fragment of two independent phage lambda clones previously isolated from a Schizophyllum genomic library. The fragment was identified by its ability to complement Schizophyllum ura1 mutants via transformation. The complete nucleotide sequence of the fragment containing the URA1 gene was determined. Sequence analysis revealed that the coding region of the URA1 gene encompasses a polypeptide of 279 amino acids (aa) interrupted by two small introns. The deduced aa sequence corresponds to 30.3 kDa and is substantially similar to the sequences of analogous polypeptides from other organisms. No canonical 5'-TATA sequence nor 3'-AATAAA polyadenylation signal are evident in the flanking regions of the URA1 gene.
担子菌裂褶菌的URA1基因（编码乳清苷-5'-单磷酸脱羧酶）被定位到两个独立的λ噬菌体克隆的一个1.4kb BglI - BamHI片段上，这两个克隆先前从裂褶菌基因组文库中分离得到。该片段通过转化互补裂褶菌ura1突变体的能力得以鉴定。测定了包含URA1基因的片段的完整核苷酸序列。序列分析表明，URA1基因的编码区包含一个由279个氨基酸（aa）组成的多肽，该多肽被两个小内含子打断。推导的氨基酸序列对应于30.3kDa，并且与来自其他生物体的类似多肽序列基本相似。在URA1基因的侧翼区域没有明显的典型5'-TATA序列和3'-AATAAA多聚腺苷酸化信号。
