利用气相色谱 - 质谱联用技术分析小鼠挥发性信息素

Analysis of volatile mouse pheromones by gas chromatography mass spectrometry.

作者信息

Novotny Milos V, Soini Helena A

机构信息

Department of Chemistry, Institute for Pheromone Research, Indiana University, Bloomington, IN, USA.

出版信息

Methods Mol Biol. 2013;1068:29-45. doi: 10.1007/978-1-62703-619-1_3.

DOI:10.1007/978-1-62703-619-1_3

PMID:24014352

Abstract

High-precision quantitative profiling of volatile organic constituents in rodent physiological fluids and glandular secretions is needed to relate olfactory signals to physiology and behavior. Whereas capillary gas chromatography-mass spectrometry (GC-MS) analysis has become the most widely applied in such investigations, the extraction and preconcentration of volatile organics is arguably the most critical step in the overall analytical task. In this chapter, we describe technical details of two main sample extraction procedures used in our laboratory: dynamic headspace trapping, and stir bar sorptive extraction (SBSE). They have been demonstrated here for the chromatographic analysis of mouse urine, serum, saliva, and preputial gland specimens.

摘要

需要对啮齿动物生理体液和腺体分泌物中的挥发性有机成分进行高精度定量分析，以便将嗅觉信号与生理和行为联系起来。尽管毛细管气相色谱 - 质谱联用（GC-MS）分析已成为此类研究中应用最广泛的方法，但挥发性有机物的提取和预浓缩可以说是整个分析任务中最关键的步骤。在本章中，我们描述了我们实验室使用的两种主要样品提取程序的技术细节：动态顶空捕集和搅拌棒吸附萃取（SBSE）。此处已展示了它们用于小鼠尿液、血清、唾液和包皮腺标本的色谱分析。