Neal Scott J, Kim Kyuhyung, Sengupta Piali
Department of Biology, Brandeis University, Waltham, MA, USA.
Methods Mol Biol. 2013;1068:273-83. doi: 10.1007/978-1-62703-619-1_20.
Environmental conditions experienced during early larval stages dictate the developmental trajectory of the nematode C. elegans. Favorable conditions such as low population density, abundant food, and lower temperatures allow reproductive growth, while stressful conditions promote entry of second-stage (L2) larvae into the alternate dauer developmental stage. Population density is signaled by the concentration and composition of a complex mixture of small molecules that is produced by all stages of animals, and is collectively referred to as dauer pheromone; pheromone concentration is a major trigger for dauer formation. Here, we describe a quantitative dauer formation assay that provides a measure of the potency of single or mixtures of pheromone components in regulating this critical developmental decision.
线虫秀丽隐杆线虫幼虫早期所经历的环境条件决定了其发育轨迹。诸如低种群密度、丰富食物和较低温度等有利条件有利于生殖生长，而应激条件则促使第二阶段（L2）幼虫进入另一种滞育发育阶段。种群密度由动物各个阶段产生的小分子复杂混合物的浓度和组成来表征，这种混合物统称为滞育信息素；信息素浓度是滞育形成的主要触发因素。在此，我们描述了一种定量滞育形成测定法，该方法可衡量信息素单一成分或混合成分在调节这一关键发育决策中的效力。
