Okamoto E, Takagi T, Azuma C, Kimura T, Tokugawa Y, Mitsuda N, Saji F, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Horm Metab Res. 1990 Jul;22(7):394-7. doi: 10.1055/s-2007-1004930.
Immunoreactive corticotropin-releasing hormone (IR-CRH) in maternal plasma increases progressively during pregnancy and decreases rapidly after delivery, suggesting that IR-CRH is produced in the placenta. We studied the expression of the CRH gene in developing human chorionic tissue, the amniotic membrane, the uterine myometrium and a fresh surgical specimen of hydatidiform mole by Northern blot analysis. Our results were as follows: (1) CRH mRNA was demonstrated in the placenta in the third trimester and at term, but under detectable level in the first and second trimesters. (2) CRH mRNA expression was observed in the amniotic membrane, but its expression in the myometrium in normal pregnancy was under detectable level at term. (3) CRH mRNA was also under detectable level in trophoblasts of a hydatidiform mole. These results suggest that the sources of the increased level of IR-CRH in human plasma and amniotic fluid during pregnancy are the placenta and amniotic membrane, and that gene expression of placental CRH increases during pregnancy.
母体血浆中的免疫反应性促肾上腺皮质激素释放激素（IR-CRH）在孕期逐渐增加，产后迅速下降，这表明IR-CRH是由胎盘产生的。我们通过Northern印迹分析研究了促肾上腺皮质激素释放激素（CRH）基因在发育中的人绒毛膜组织、羊膜、子宫肌层及葡萄胎新鲜手术标本中的表达。结果如下：（1）CRH mRNA在妊娠晚期及足月时的胎盘中有表达，但在妊娠早期和中期低于可检测水平。（2）在羊膜中观察到CRH mRNA表达，但足月时正常妊娠子宫肌层中的表达低于可检测水平。（3）葡萄胎滋养层细胞中的CRH mRNA也低于可检测水平。这些结果表明，孕期人血浆和羊水中IR-CRH水平升高的来源是胎盘和羊膜，且胎盘CRH的基因表达在孕期增加。
